usp10 inhibitor spautin Search Results


usp10 sirna  (Beyotime)
90
Beyotime usp10 sirna
<t>USP10</t> regulates the levels of p53 in IQC-treated cells under HG stress. (A) Representative immunofluorescence images of p53 and USP10 co-localization. Fluorescence was evaluated by confocal microscopy. p53 (green), USP10 (red), DAPI (blue). Scale bar, 20 µ m. (B) Representative western blotting images of p53, USP10 and GAPDH protein bands in HUVECs transfected with <t>siUSP10</t> or NC siRNA and their quantification (n=3). (C) Representative western blotting images of USP10, p53 and GAPDH protein bands in HUVECs infected with adUSP10 or adGFP and their quantification (n=3). * P<0.05 and ** P<0.01 vs. NC or adGFP. # P<0.05 and ## P<0.01 vs. IQC+NC or IQC+adGFP. NG, normal glucose; HG, high glucose; IQC, isoquercitrin; HUVECs, human umbilical vein endothelial cells; si, small interfering; NC, negative control; USP10, ubiquitin specific peptidase 10; ad, adenovirus.
Usp10 Sirna, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
usp10 sirna - by Bioz Stars, 2026-02
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USP10 regulates the levels of p53 in IQC-treated cells under HG stress. (A) Representative immunofluorescence images of p53 and USP10 co-localization. Fluorescence was evaluated by confocal microscopy. p53 (green), USP10 (red), DAPI (blue). Scale bar, 20 µ m. (B) Representative western blotting images of p53, USP10 and GAPDH protein bands in HUVECs transfected with siUSP10 or NC siRNA and their quantification (n=3). (C) Representative western blotting images of USP10, p53 and GAPDH protein bands in HUVECs infected with adUSP10 or adGFP and their quantification (n=3). * P<0.05 and ** P<0.01 vs. NC or adGFP. # P<0.05 and ## P<0.01 vs. IQC+NC or IQC+adGFP. NG, normal glucose; HG, high glucose; IQC, isoquercitrin; HUVECs, human umbilical vein endothelial cells; si, small interfering; NC, negative control; USP10, ubiquitin specific peptidase 10; ad, adenovirus.

Journal: International Journal of Molecular Medicine

Article Title: Isoquercitrin protects HUVECs against high glucose-induced apoptosis through regulating p53 proteasomal degradation

doi: 10.3892/ijmm.2021.4955

Figure Lengend Snippet: USP10 regulates the levels of p53 in IQC-treated cells under HG stress. (A) Representative immunofluorescence images of p53 and USP10 co-localization. Fluorescence was evaluated by confocal microscopy. p53 (green), USP10 (red), DAPI (blue). Scale bar, 20 µ m. (B) Representative western blotting images of p53, USP10 and GAPDH protein bands in HUVECs transfected with siUSP10 or NC siRNA and their quantification (n=3). (C) Representative western blotting images of USP10, p53 and GAPDH protein bands in HUVECs infected with adUSP10 or adGFP and their quantification (n=3). * P<0.05 and ** P<0.01 vs. NC or adGFP. # P<0.05 and ## P<0.01 vs. IQC+NC or IQC+adGFP. NG, normal glucose; HG, high glucose; IQC, isoquercitrin; HUVECs, human umbilical vein endothelial cells; si, small interfering; NC, negative control; USP10, ubiquitin specific peptidase 10; ad, adenovirus.

Article Snippet: To knock down USP10 expression, HUVECs were seeded in 6-well plates, grown in antibiotic-free HG medium for 48 h and then transfected with USP10 siRNA (siUSP10; position 1583-1601; 5′-CCACCTGATGAAGTTCATT-3′) and non-targeting NC siRNA using Lipofectamine ® 6000 (Beyotime Institute of Biotechnology) at a final concentration of 100 nM at 37°C for 6 h according to the manufacturer's instructions.

Techniques: Immunofluorescence, Fluorescence, Confocal Microscopy, Western Blot, Transfection, Infection, Negative Control, Ubiquitin Proteomics

Chemical structure of isoquercitrin and the proposed mechanisms of the protective effect of isoquercitrin in endothelial cells. (A) Structure of isoquercitrin. (B) Isoquercitrin modulates HG-induced apoptosis through a p53-medicated signaling pathway in endothelial cells. USP10, ubiquitin specific peptidase 10; p, phosphorylated; C-caspase 3, cleaved caspase 3.

Journal: International Journal of Molecular Medicine

Article Title: Isoquercitrin protects HUVECs against high glucose-induced apoptosis through regulating p53 proteasomal degradation

doi: 10.3892/ijmm.2021.4955

Figure Lengend Snippet: Chemical structure of isoquercitrin and the proposed mechanisms of the protective effect of isoquercitrin in endothelial cells. (A) Structure of isoquercitrin. (B) Isoquercitrin modulates HG-induced apoptosis through a p53-medicated signaling pathway in endothelial cells. USP10, ubiquitin specific peptidase 10; p, phosphorylated; C-caspase 3, cleaved caspase 3.

Article Snippet: To knock down USP10 expression, HUVECs were seeded in 6-well plates, grown in antibiotic-free HG medium for 48 h and then transfected with USP10 siRNA (siUSP10; position 1583-1601; 5′-CCACCTGATGAAGTTCATT-3′) and non-targeting NC siRNA using Lipofectamine ® 6000 (Beyotime Institute of Biotechnology) at a final concentration of 100 nM at 37°C for 6 h according to the manufacturer's instructions.

Techniques: Ubiquitin Proteomics